Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

MITOL-dependent ubiquitylation negatively regulates the entry of Pol?A into mitochondria.

Hussain M, Mohammed A, Saifi S, Khan A, Kaur E, Priya S, Agarwal H, Sengupta S

Mutations in mitochondrial replicative polymerase Pol?A lead to progressive external ophthalmoplegia (PEO). While Pol?A is the known central player in mitochondrial DNA (mtDNA) replication, it is unknown whether a regulatory process exists on the mitochondrial outer membrane which controlled its entry into the mitochondria. We now demonstrate that Pol?A is ubiquitylated by mitochondrial E3 ligase, MITOL (or MARCH5, RNF153). Ubiquitylation ... [more]

PLoS Biol Dec. 01, 2020; 19(3);e3001139 [Pubmed: 33657094]

Throughput

  • Low Throughput

Curated By

  • BioGRID