AGO2
Gene Ontology Biological Process
- Fc-epsilon receptor signaling pathway [TAS]
- Notch signaling pathway [TAS]
- RNA phosphodiester bond hydrolysis, endonucleolytic [EXP, IDA]
- epidermal growth factor receptor signaling pathway [TAS]
- fibroblast growth factor receptor signaling pathway [TAS]
- gene expression [TAS]
- gene silencing by RNA [ISS]
- innate immune response [TAS]
- mRNA cleavage involved in gene silencing by miRNA [IDA, IMP]
- negative regulation of translation involved in gene silencing by miRNA [IDA, IMP]
- negative regulation of translational initiation [IDA]
- neurotrophin TRK receptor signaling pathway [TAS]
- phosphatidylinositol-mediated signaling [TAS]
- positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay [ISS]
- positive regulation of nuclear-transcribed mRNA poly(A) tail shortening [ISS]
- pre-miRNA processing [IDA]
- translation [NAS]
- translational initiation [NAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
EIF4B
Gene Ontology Biological Process
- RNA metabolic process [TAS]
- cellular protein metabolic process [TAS]
- gene expression [TAS]
- insulin receptor signaling pathway [TAS]
- mRNA metabolic process [TAS]
- nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay [TAS]
- nuclear-transcribed mRNA poly(A) tail shortening [TAS]
- regulation of translational initiation [TAS]
- translation [TAS]
- translational initiation [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Proteomic and functional analysis of Argonaute-containing mRNA-protein complexes in human cells.
Members of the Argonaute (Ago) protein family associate with small RNAs and have important roles in RNA silencing. Here, we analysed Ago1- and Ago2-containing protein complexes in human cells. Separation of Ago-associated messenger ribonucleoproteins (mRNPs) showed that Ago1 and Ago2 reside in three complexes with distinct Dicer and RNA-induced silencing complex activities. A comprehensive proteomic analysis of Ago-containing mRNPs identified ... [more]
Throughput
- High Throughput
Curated By
- BioGRID