OPTN
Gene Ontology Biological Process
- G2/M transition of mitotic cell cycle [TAS]
- Golgi organization [IMP]
- Golgi ribbon formation [IDA]
- Golgi to plasma membrane protein transport [IMP]
- cell death [TAS]
- defense response to Gram-negative bacterium [IMP]
- macroautophagy [IDA]
- mitotic cell cycle [TAS]
- negative regulation of receptor recycling [IMP]
- protein targeting to Golgi [IMP]
- regulation of I-kappaB kinase/NF-kappaB signaling [IBA]
- signal transduction [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
RAB8A
Gene Ontology Biological Process
- G2/M transition of mitotic cell cycle [TAS]
- GTP catabolic process [IBA, IDA]
- Golgi vesicle fusion to target membrane [IDA]
- Rab protein signal transduction [IBA]
- axonogenesis [ISS]
- cellular response to insulin stimulus [IBA, ISS]
- cilium assembly [IDA, IMP]
- intracellular protein transport [IBA]
- membrane organization [TAS]
- mitotic cell cycle [TAS]
- protein localization to plasma membrane [IBA, ISS]
- protein secretion [IBA]
- regulation of exocytosis [IBA]
- synaptic vesicle exocytosis [IBA]
- vesicle docking involved in exocytosis [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Golgi membrane [TAS]
- centrosome [IDA]
- cilium [IDA]
- cytoplasmic vesicle membrane [TAS]
- extracellular vesicular exosome [IDA]
- nonmotile primary cilium [IDA]
- nucleolus [IDA]
- nucleoplasm [IDA]
- nucleus [IDA]
- phagocytic vesicle [IDA]
- plasma membrane [IBA, IDA]
- primary cilium [IDA]
- recycling endosome membrane [IDA]
- secretory granule membrane [IBA]
- synaptic vesicle [IBA]
- trans-Golgi network transport vesicle [IBA]
Reconstituted Complex
An interaction is detected between purified proteins in vitro.
Publication
FIP-2, a coiled-coil protein, links Huntingtin to Rab8 and modulates cellular morphogenesis.
Huntington's disease is characterised by the death of cortical and striatal neurons, and is the result of an expanded polyglutamine tract in the Huntingtin protein [1]. Huntingtin is present on both endocytic and secretory membrane organelles but its function is unclear [2,3]. Rab GTPases regulate both of these transport pathways [4]. We have previously shown that Rab8 controls polarised membrane ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| OPTN RAB8A | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| RAB8A OPTN | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| OPTN RAB8A | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| OPTN RAB8A | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| OPTN RAB8A | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - | |
| RAB8A OPTN | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID