BAIT

PKC1

CLY15, CLY5, CLY7, HPO2, STT1, protein kinase C, L000001446, L000000362, S000029091, YBL105C
Protein serine/threonine kinase; essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)
Kinome Project (Sc)
Saccharomyces cerevisiae (S288c)
PREY

ATG15

AUT5, CVT17, triglyceride lipase ATG15, L000004767, L000004751, YCR068W
Lipase required for intravacuolar lysis of autophagic and Cvt bodies; targeted to intravacuolar vesicles during autophagy via the multivesicular body (MVB) pathway
Saccharomyces cerevisiae (S288c)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

The synthetic genetic network around PKC1 identifies novel modulators and components of protein kinase C signaling in Saccharomyces cerevisiae.

Krause SA, Xu H, Gray JV

Budding yeast Saccharomyces cerevisiae contains one protein kinase C (PKC) isozyme encoded by the essential gene PKC1. Pkc1 is activated by the small GTPase Rho1 and plays a central role in the cell wall integrity (CWI) signaling pathway. This pathway acts primarily to remodel the cell surface throughout the normal life cycle and upon various environmental stresses. The pathway is ... [more]

Eukaryotic Cell Nov. 01, 2008; 7(11);1880-7 [Pubmed: 18806213]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: inviable (APO:0000112)

Additional Notes

  • A dominant-negative SGA (DN-SGA) analysis was performed. The wild-type query strain was transformed with a plasmid containing a dominant-negative allele of PKC1 encoding a catalytically inactive full-length Pkc1 protein

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PKC1 ATG15
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.1594BioGRID
1959329

Curated By

  • BioGRID