BAIT

PKC1

CLY15, CLY5, CLY7, HPO2, STT1, protein kinase C, L000001446, L000000362, S000029091, YBL105C
Protein serine/threonine kinase; essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)
Kinome Project (Sc)
Saccharomyces cerevisiae (S288c)
PREY

CSF1

YLR087C
Protein required for fermentation at low temperature; plays a role in the maturation of secretory proteins; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
GO Process (2)
GO Function (0)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

The synthetic genetic network around PKC1 identifies novel modulators and components of protein kinase C signaling in Saccharomyces cerevisiae.

Krause SA, Xu H, Gray JV

Budding yeast Saccharomyces cerevisiae contains one protein kinase C (PKC) isozyme encoded by the essential gene PKC1. Pkc1 is activated by the small GTPase Rho1 and plays a central role in the cell wall integrity (CWI) signaling pathway. This pathway acts primarily to remodel the cell surface throughout the normal life cycle and upon various environmental stresses. The pathway is ... [more]

Eukaryotic Cell Nov. 01, 2008; 7(11);1880-7 [Pubmed: 18806213]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: inviable (APO:0000112)

Additional Notes

  • A dominant-negative SGA (DN-SGA) analysis was performed. The wild-type query strain was transformed with a plasmid containing a dominant-negative allele of PKC1 encoding a catalytically inactive full-length Pkc1 protein

Curated By

  • BioGRID