BAIT
PKC1
CLY15, CLY5, CLY7, HPO2, STT1, protein kinase C, L000001446, L000000362, S000029091, YBL105C
Protein serine/threonine kinase; essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)
GO Process (8)
GO Function (1)
GO Component (6)
Gene Ontology Biological Process
- actin filament organization [IGI]
- cytoplasmic mRNA processing body assembly [IMP]
- intracellular signal transduction [IMP]
- peroxisome degradation [IMP]
- protein phosphorylation [IDA]
- regulation of fungal-type cell wall organization [IMP]
- regulation of nuclear-transcribed mRNA poly(A) tail shortening [IMP]
- signal transduction [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
SEC28
ANU2, L000004402, YIL076W
Epsilon-COP subunit of the coatomer; regulates retrograde Golgi-to-ER protein traffic; stabilizes Cop1p, the alpha-COP and the coatomer complex; non-essential for cell growth; protein abundance increases in response to DNA replication stress
GO Process (3)
GO Function (0)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Synthetic Lethality
A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.
Publication
The synthetic genetic network around PKC1 identifies novel modulators and components of protein kinase C signaling in Saccharomyces cerevisiae.
Budding yeast Saccharomyces cerevisiae contains one protein kinase C (PKC) isozyme encoded by the essential gene PKC1. Pkc1 is activated by the small GTPase Rho1 and plays a central role in the cell wall integrity (CWI) signaling pathway. This pathway acts primarily to remodel the cell surface throughout the normal life cycle and upon various environmental stresses. The pathway is ... [more]
Eukaryotic Cell Nov. 01, 2008; 7(11);1880-7 [Pubmed: 18806213]
Throughput
- Low Throughput
Ontology Terms
- phenotype: inviable (APO:0000112)
Additional Notes
- A dominant-negative SGA (DN-SGA) analysis was performed. The wild-type query strain was transformed with a plasmid containing a dominant-negative allele of PKC1 encoding a catalytically inactive full-length Pkc1 protein
Curated By
- BioGRID