Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

The Cdk-like protein PCTAIRE-1 from mouse brain associates with p11 and 14-3-3 proteins.

Sladeczek F, Camonis JH, Burnol AF, Le Bouffant F

PCTAIRE-1 is a member of the cyclin-dependent kinase (cdk)-like class of proteins, and is localized mainly in the mammalian brain. Using the yeast two-hybrid system we screened a mouse brain cDNA library with PCTAIRE-1 as bait, and isolated several clones coding for the mouse homologs of the following proteins: p11 (also known as calpactin I light chain) and the eta, ... [more]

Mol. Gen. Genet. May. 20, 1997; 254(5);571-7 [Pubmed: 9197417]

Throughput

  • Low Throughput

Curated By

  • BioGRID