Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Differential ability to form the G protein betagamma complex among members of the beta and gamma subunit families.

Yan K, Kalyanaraman V, Gautam N

We have determined the relative abilities of several members of the G protein beta and gamma subunit families to associate with each other using the yeast two-hybrid system. We show first that the mammalian beta1 and gamma3 fusion proteins form a complex in yeast and that formation of the complex activates the reporter gene for beta-galactosidase. Second, the magnitude of ... [more]

J. Biol. Chem. Mar. 22, 1996; 271(12);7141-6 [Pubmed: 8636150]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
GNG3 GNB4	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2017)	High	1	BioGRID	2225056
GNG3 GNB4	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2021)	High	0.9997	BioGRID	3066523
GNB4 GNG3	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Rosskopf D (2003)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

GNG3

Gene Ontology Biological Process

Gene Ontology Molecular Function

GTPase activity [TAS]

Gene Ontology Cellular Component

GNB4