BAIT

GNG3

guanine nucleotide binding protein (G protein), gamma 3
GO Process (6)
GO Function (1)
GO Component (1)
Homo sapiens
PREY

GNB4

CMTD1F, RP11-145M9.4
guanine nucleotide binding protein (G protein), beta polypeptide 4
GO Process (4)
GO Function (1)
GO Component (3)
Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Differential ability to form the G protein betagamma complex among members of the beta and gamma subunit families.

Yan K, Kalyanaraman V, Gautam N

We have determined the relative abilities of several members of the G protein beta and gamma subunit families to associate with each other using the yeast two-hybrid system. We show first that the mammalian beta1 and gamma3 fusion proteins form a complex in yeast and that formation of the complex activates the reporter gene for beta-galactosidase. Second, the magnitude of ... [more]

J. Biol. Chem. Mar. 22, 1996; 271(12);7141-6 [Pubmed: 8636150]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
GNG3 GNB4
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High1BioGRID
2225056
GNG3 GNB4
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.9997BioGRID
3066523
GNB4 GNG3
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
-

Curated By

  • BioGRID