BAIT

IL1RAP

6430709H04Rik, AI255955, AV239853, IL-1RAcP
interleukin 1 receptor accessory protein
GO Process (2)
GO Function (4)
GO Component (3)
Mus musculus
PREY

IRAK1

AA408924, IRAK, IRAK-1, IRAK1-S, Il1rak, Plpk, mPLK, RP23-252M19.7
interleukin-1 receptor-associated kinase 1
GO Process (23)
GO Function (8)
GO Component (5)
Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Transient membrane recruitment of IRAK-1 in response to LPS and IL-1beta requires TNF R1.

Lockett A, Goebl MG, Harrington MA

The transcription factor NF-kappaB is an essential regulator of the innate immune response that functions as the first line of defense against infections. Activation of the innate immune response by bacterial lipopolysaccharide (LPS) triggers production of tumor necrosis factor-alpha (TNF-alpha) followed by interleukin-1 (IL-1). The IL-1 receptor associated kinase-1 (IRAK-1) is an integral component of the LPS, TNF-alpha, and IL-1 ... [more]

Am. J. Physiol., Cell Physiol. Aug. 01, 2008; 295(2);C313-23 [Pubmed: 18562480]

Throughput

  • Low Throughput

Curated By

  • BioGRID