BAIT

CCNF

FBX1, FBXO1

cyclin F

GO Process (3)

GO Function (1)

GO Component (3)

Gene Ontology Biological Process

SCF-dependent proteasomal ubiquitin-dependent protein catabolic process [IDA]

negative regulation of centrosome duplication [IDA]

protein ubiquitination [IDA]

Gene Ontology Molecular Function

protein binding [IPI]

Gene Ontology Cellular Component

SCF ubiquitin ligase complex [IDA]

centriole [IDA]

CRISPR Database HGNC Alliance of Genome Resources VEGA OMIM Entrez Gene RefSeq UniprotKB Ensembl HPRD

Homo sapiens

PREY

HSPB1

CMT2F, HEL-S-102, HMN2B, HS.76067, HSP27, HSP28, Hsp25, SRP27

heat shock 27kDa protein 1

Alzheimer's Disease Project

GO Process (21)

GO Function (7)

GO Component (8)

Gene Ontology Biological Process

RNA metabolic process [TAS]

cellular component movement [TAS]

cellular response to vascular endothelial growth factor stimulus [IMP]

gene expression [TAS]

intracellular signal transduction [IMP]

mRNA metabolic process [TAS]

negative regulation of apoptotic process [TAS]

negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway [ISS]

negative regulation of protein kinase activity [ISS]

platelet aggregation [IMP]

positive regulation of angiogenesis [IMP]

positive regulation of blood vessel endothelial cell migration [IMP]

positive regulation of endothelial cell chemotaxis [IMP]

positive regulation of endothelial cell chemotaxis by VEGF-activated vascular endothelial growth factor receptor signaling pathway [IMP]

positive regulation of interleukin-1 beta production [ISS]

positive regulation of tumor necrosis factor biosynthetic process [ISS]

regulation of I-kappaB kinase/NF-kappaB signaling [ISS]

regulation of translational initiation [TAS]

response to unfolded protein [NAS]

response to virus [IEP]

retina homeostasis [IEP]

Gene Ontology Molecular Function

identical protein binding [IPI]
poly(A) RNA binding [IDA]
protein binding [IPI]
protein kinase C binding [ISS]
protein kinase C inhibitor activity [ISS]
protein kinase binding [IPI]
ubiquitin binding [ISS]

Gene Ontology Cellular Component

cytoplasm [IDA, TAS]

cytoskeleton [TAS]

extracellular space [IDA]

extracellular vesicular exosome [IDA]

focal adhesion [IDA]

proteasome complex [ISS]

CRISPR Database OMIM HGNC Alliance of Genome Resources VEGA Entrez Gene RefSeq UniprotKB Ensembl HPRD

Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

ALS/FTD-causing mutation in cyclin F causes the dysregulation of SFPQ.

Rayner SL, Cheng F, Hogan AL, Grima N, Yang S, Ke YD, Au CG, Morsch M, De Luca A, Davidson JM, Molloy MP, Shi B, Ittner LM, Blair I, Chung RS, Lee A

Previously, we identified missense mutations in CCNF that are causative of familial and sporadic amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). Hallmark features of these diseases include the build-up of insoluble protein aggregates as well as the mislocalization of proteins such as transactive response DNA binding protein 43 kDa (TDP-43). In recent years, the dysregulation of SFPQ (splicing factor ... [more]

Hum Mol Genet May. 31, 2021; 30(11);971-984 [Pubmed: 33729478]

Quantitative Score

19.812 [Confidence Score]

Throughput

High Throughput

Additional Notes

Affinity Capture-MS was carried out to identify high-confidence protein interactions with an abundance ratio that was >1.5 fold over controls (Fold Abundance ratio over vector control reported)

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
CCNF HSPB1	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	Siebert A (2022)	High	-	BioGRID	-

Curated By

BioGRID