BAIT

HIF1A

HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78
hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
GO Process (34)
GO Function (13)
GO Component (7)

Gene Ontology Biological Process

Homo sapiens
PREY

VHL

Vhlh
von Hippel-Lindau tumor suppressor, E3 ubiquitin protein ligase
Rattus norvegicus

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Hypoxia-inducible factor-1alpha stabilization in nonhypoxic conditions: role of oxidation and intracellular ascorbate depletion.

Page EL, Chan DA, Giaccia AJ, Levine M, Richard DE

Hypoxia-inducible factor-1 (HIF-1) is a decisive element for the transcriptional regulation of many genes induced under low oxygen conditions. Under normal oxygen conditions, HIF-1alpha, the active subunit of HIF-1, is hydroxylated on proline residues by specific HIF prolyl-hydroxylases, leading to ubiquitination and degradation by the proteasome. In hypoxia, hydroxylation and ubiquitination are blocked and HIF-1alpha accumulates in cells. Recent studies ... [more]

Mol. Biol. Cell Jan. 01, 2008; 19(1);86-94 [Pubmed: 17942596]

Throughput

  • Low Throughput

Additional Notes

  • Hif1a not confirmed human

Curated By

  • BioGRID