An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Human transcription factor protein interaction networks

Goos H, Kinnunen M, Salokas K, Tan Z, Liu X, Yadav L, Zhang Q, Wei G-H, Varjosalo M

Transcription factors (TFs) interact with several other proteins in the process of transcriptional regulation. Here, we identify 6703 and 1536 protein-protein interactions for 109 different human TFs through proximity-dependent biotinylation (BioID) and affinity purification mass spectrometry (AP-MS), respectively. The BioID analysis identifies more high-confidence interactions, highlighting the transient and dynamic nature of many of the TF interactions. By performing clustering ... [more]

Nature Communications Feb. 09, 2022; (); [Pubmed: 35140242]

Throughput

High Throughput

Additional Notes

BioID

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
ERG PIAS1	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Viard J (2022)	High	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

ERG

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA binding [TAS]protein binding [IPI]sequence-specific DNA binding RNA polymerase II transcription factor activity [IBA]signal transducer activity [TAS]

Gene Ontology Cellular Component

PIAS1

Gene Ontology Biological Process

Gene Ontology Molecular Function

SUMO transferase activity [IDA]androgen receptor binding [NAS]enzyme binding [IPI]protein binding [IPI]transcription coactivator activity [NAS]transcription corepressor activity [TAS]