USP5
Gene Ontology Biological Process
Gene Ontology Molecular Function
NLRP3
Gene Ontology Biological Process
- NLRP3 inflammasome complex assembly [IMP]
- activation of cysteine-type endopeptidase activity involved in apoptotic process [IMP]
- cellular response to lipopolysaccharide [ISO]
- defense response to virus [IMP]
- inflammatory response [ISO]
- interleukin-1 beta production [IMP]
- interleukin-1 secretion [IMP]
- interleukin-18 production [IMP]
- negative regulation of NF-kappaB import into nucleus [ISO]
- negative regulation of NF-kappaB transcription factor activity [ISO]
- negative regulation of acute inflammatory response [ISO]
- negative regulation of inflammatory response [ISO]
- negative regulation of interleukin-1 beta secretion [ISO]
- positive regulation of NF-kappaB transcription factor activity [ISO]
- positive regulation of cysteine-type endopeptidase activity involved in apoptotic process [ISO]
- positive regulation of interleukin-1 beta secretion [IGI, ISO]
- regulation of inflammatory response [IMP]
Gene Ontology Molecular Function
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
USP5 attenuates NLRP3 inflammasome activation by promoting autophagic degradation of NLRP3.
3-MA: 3-methyladenine; AIM2: absent in melanoma 2; ATG5: autophagy related 5; BafA1: bafilomycin A1; CASP1: caspase 1; CHX: cycloheximide; Co-IP: co-immunoprecipitation; CQ: chloroquine; DUBs: deubiquitinases; IL1B/IL-1?: interleukin 1 beta; LAMP1: lysosomal associated membrane protein 1; LPS: lipopolysaccharide; MARCHF7/MARCH7: membrane associated RING-CH-type finger 7; NFKB/NF-?B: nuclear factor kappa B; Nig.: nigericin; NLRC4: NLR family CARD domain containing 4; NLRP3: NLR family ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID