CDC25B
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- centrosome [IDA]
- cytoplasm [IDA]
- cytosol [TAS]
- nucleoplasm [IDA, TAS]
- spindle pole [IDA]
CCNB1
Gene Ontology Biological Process
- G1/S transition of mitotic cell cycle [TAS]
- G2/M transition of mitotic cell cycle [TAS]
- anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process [TAS]
- mitotic cell cycle [TAS]
- mitotic metaphase plate congression [IMP]
- mitotic nuclear envelope disassembly [TAS]
- mitotic spindle stabilization [IMP]
- positive regulation of attachment of spindle microtubules to kinetochore [IMP]
- positive regulation of mitotic cell cycle [IMP]
- positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [TAS]
- regulation of cell cycle [TAS]
- regulation of mitotic cell cycle spindle assembly checkpoint [IMP]
- regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Phosphorylation of Cdc25B3 Ser169 regulates 14-3-3 binding to Ser151 and Cdc25B activity.
Cdc25B is a major regulator of G(2) phase progression into mitosis and in recovery from a G(2) phase checkpoint arrest. The activity of Cdc25B is regulated by 14-3-3 dimer binding to a high-affinity phosphosite Ser323 and two lower-affinity phosphosites Ser151 and Ser230, which blocks substrate access to the catalytic site and, thereby, activity. Loss of 14-3-3 binding to any of ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| CDC25B CCNB1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 2215328 |
Curated By
- BioGRID