BAIT

BRN1

L000004324, YBL097W
Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission
GO Process (4)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

TOP2

TOR3, TRF3, DNA topoisomerase 2, L000002320, YNL088W
Topoisomerase II; relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis; required for replication slow zone (RSZ) breakage following Mec1p inactivation
Saccharomyces cerevisiae (S288c)

Co-localization

Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.

Publication

Condensin-dependent rDNA decatenation introduces a temporal pattern to chromosome segregation.

D'Ambrosio C, Kelly G, Shirahige K, Uhlmann F

The chromosomal condensin complex gives metaphase chromosomes structural stability. In addition, condensin is required for sister-chromatid resolution during their segregation in anaphase [1-7]. How condensin promotes chromosome resolution is poorly understood. Chromosome segregation during anaphase also fails after inactivation of topoisomerase II (topo II), the enzyme that removes catenation between sister chromatids left behind after completion of DNA replication [8, ... [more]

Curr. Biol. Jul. 22, 2008; 18(14);1084-9 [Pubmed: 18635352]

Throughput

  • Low Throughput

Additional Notes

  • ChIP

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
TOP2 BRN1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.5127BioGRID
1948546

Curated By

  • BioGRID