BAIT
RAC3
ras-related C3 botulinum toxin substrate 3 (rho family, small GTP binding protein Rac3)
GO Process (8)
GO Function (3)
GO Component (10)
Gene Ontology Biological Process
- GTP catabolic process [IDA]
- actin cytoskeleton organization [IDA]
- cell projection assembly [IDA]
- intracellular signal transduction [TAS]
- positive regulation of cell adhesion mediated by integrin [IDA]
- positive regulation of substrate adhesion-dependent cell spreading [IDA]
- regulation of small GTPase mediated signal transduction [TAS]
- small GTPase mediated signal transduction [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
PIK3CA
CLOVE, CWS5, MCAP, MCM, MCMTC, PI3K, p110-alpha
phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha
GO Process (22)
GO Function (6)
GO Component (4)
Gene Ontology Biological Process
- Fc-epsilon receptor signaling pathway [TAS]
- Fc-gamma receptor signaling pathway involved in phagocytosis [TAS]
- T cell costimulation [TAS]
- T cell receptor signaling pathway [TAS]
- blood coagulation [TAS]
- cardiac muscle contraction [TAS]
- endothelial cell migration [TAS]
- epidermal growth factor receptor signaling pathway [TAS]
- fibroblast growth factor receptor signaling pathway [TAS]
- innate immune response [TAS]
- insulin receptor signaling pathway [TAS]
- insulin receptor signaling pathway via phosphatidylinositol 3-kinase [TAS]
- leukocyte migration [TAS]
- negative regulation of anoikis [IMP]
- neurotrophin TRK receptor signaling pathway [TAS]
- phosphatidylinositol biosynthetic process [TAS]
- phosphatidylinositol phosphorylation [ISS]
- phosphatidylinositol-mediated signaling [TAS]
- phospholipid metabolic process [TAS]
- platelet activation [TAS]
- small molecule metabolic process [TAS]
- vasculature development [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Mapping the proximity interaction network of the Rho-family GTPases reveals signalling pathways and regulatory mechanisms.
Guanine nucleotide exchange factors (RhoGEFs) and GTPase-activating proteins (RhoGAPs) coordinate the activation state of the Rho family of GTPases for binding to effectors. Here, we exploited proximity-dependent biotinylation to systematically define the Rho family proximity interaction network from 28 baits to produce 9,939 high-confidence proximity interactions in two cell lines. Exploiting the nucleotide states of Rho GTPases, we revealed the ... [more]
Nat. Cell Biol. Dec. 23, 2019; (); [Pubmed: 31871319]
Throughput
- High Throughput
Additional Notes
- AvgP greater or equal to 0.95 assessed by SAINT express
- BioID
- RAC3-G12V active mutant bait, interaction in HEK293 cells
Curated By
- BioGRID