Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

PRAMEF2-mediated dynamic regulation of YAP signaling promotes tumorigenesis.

Ghosh M, Das S

PRAMEF2 is a member of the PRAME multigene family of cancer testis antigens, which serve as prognostic markers for several cancers. However, molecular mechanisms underlying its role in tumorigenesis remain poorly understood. Here, we report that PRAMEF2 is repressed under conditions of altered metabolic homeostasis in a FOXP3-dependent manner. We further demonstrate that PRAMEF2 is a BC-box containing substrate recognition ... [more]

Proc Natl Acad Sci U S A Dec. 05, 2020; 118(40); [Pubmed: 34593639]

Throughput

  • High Throughput

Curated By

  • BioGRID