BAIT

AHP1

histidine-containing phosphotransmitter 1, AT3G21510
histidine-containing phosphotransmitter 1
Arabidopsis thaliana (Columbia)
PREY

ARR9

ATRR4, F24I3_120, RESPONSE REGULATOR 4, response regulator 9, AT3G57040
two-component response regulator ARR9
GO Process (5)
GO Function (2)
GO Component (1)
Arabidopsis thaliana (Columbia)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Possible His to Asp phosphorelay signaling in an Arabidopsis two-component system.

Urao T, Miyata S, Yamaguchi-Shinozaki K, Shinozaki K

We have so far cloned a cDNA encoding a hybrid-type histidine kinase (ATHK1), three cDNAs encoding phosphorelay intermediates (ATHP1-3), and four cDNAs encoding response regulators (ATRR1-4) from Arabidopsis thaliana. To determine which molecules constitute a His to Asp phosphorelay pathway, we examined protein-protein interactions between them using a pairwise yeast two-hybrid analysis, as an initial step. We detected a specific ... [more]

FEBS Lett. Aug. 04, 2000; 478(3);227-32 [Pubmed: 10930573]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
AHP1 ARR9
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-
ARR9 AHP1
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-
ARR9 AHP1
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID