BAIT
ATP2B1
PMCA1, PMCA1kb
ATPase, Ca++ transporting, plasma membrane 1
GO Process (4)
GO Function (1)
GO Component (5)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
ACSL3
ACS3, FACL3, PRO2194
acyl-CoA synthetase long-chain family member 3
GO Process (6)
GO Function (3)
GO Component (5)
Gene Ontology Biological Process
- long-chain fatty acid import [IDA]
- long-chain fatty acid metabolic process [IMP]
- positive regulation of Golgi to plasma membrane protein transport [IMP]
- positive regulation of phosphatidylcholine biosynthetic process [IMP]
- positive regulation of secretion [IMP]
- very-low-density lipoprotein particle assembly [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
OpenCell: Endogenous tagging for the cartography of human cellular organization.
Elucidating the wiring diagram of the human cell is a central goal of the postgenomic era. We combined genome engineering, confocal live-cell imaging, mass spectrometry, and data science to systematically map the localization and interactions of human proteins. Our approach provides a data-driven description of the molecular and spatial networks that organize the proteome. Unsupervised clustering of these networks delineates ... [more]
Science Dec. 11, 2021; 375(6585);eabi6983 [Pubmed: 35271311]
Throughput
- High Throughput
Additional Notes
- Bait generated from library of CRISPR-edited human embryonic kidney (HEK) 293T cell lines harboring fluorescent tags on individual proteins
Curated By
- BioGRID