BAIT
CANX
CNX, IP90, P90
calnexin
GO Process (9)
GO Function (2)
GO Component (7)
Gene Ontology Biological Process
- antigen processing and presentation of exogenous peptide antigen via MHC class II [TAS]
- antigen processing and presentation of peptide antigen via MHC class I [TAS]
- cellular protein metabolic process [TAS]
- clathrin-mediated endocytosis [ISS]
- post-translational protein modification [TAS]
- protein N-linked glycosylation via asparagine [TAS]
- protein folding [TAS]
- protein secretion [TAS]
- synaptic vesicle endocytosis [ISS]
Gene Ontology Molecular Function
Homo sapiens
PREY
SLC1A4
ASCT1, SATT
solute carrier family 1 (glutamate/neutral amino acid transporter), member 4
GO Process (15)
GO Function (8)
GO Component (9)
Gene Ontology Biological Process
- L-alanine transport [IDA]
- L-cystine transport [IDA]
- L-serine transport [IDA]
- amino acid transport [TAS]
- chloride transmembrane transport [IDA]
- chloride transport [IDA]
- cognition [IMP]
- glutamine transport [TAS]
- hydroxyproline transport [IDA]
- ion transport [TAS]
- proline transmembrane transport [IDA]
- proline transport [IDA]
- synaptic transmission, glutamatergic [NAS]
- threonine transport [IDA]
- transmembrane transport [TAS]
Gene Ontology Molecular Function- L-alanine transmembrane transporter activity [IDA]
- L-cystine transmembrane transporter activity [IDA]
- L-glutamine transmembrane transporter activity [TAS]
- L-hydroxyproline transmembrane transporter activity [IDA]
- L-proline transmembrane transporter activity [IDA]
- L-serine transmembrane transporter activity [IDA]
- L-threonine transmembrane transporter activity [IDA]
- chloride channel activity [IDA]
- L-alanine transmembrane transporter activity [IDA]
- L-cystine transmembrane transporter activity [IDA]
- L-glutamine transmembrane transporter activity [TAS]
- L-hydroxyproline transmembrane transporter activity [IDA]
- L-proline transmembrane transporter activity [IDA]
- L-serine transmembrane transporter activity [IDA]
- L-threonine transmembrane transporter activity [IDA]
- chloride channel activity [IDA]
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
OpenCell: Endogenous tagging for the cartography of human cellular organization.
Elucidating the wiring diagram of the human cell is a central goal of the postgenomic era. We combined genome engineering, confocal live-cell imaging, mass spectrometry, and data science to systematically map the localization and interactions of human proteins. Our approach provides a data-driven description of the molecular and spatial networks that organize the proteome. Unsupervised clustering of these networks delineates ... [more]
Science Dec. 11, 2021; 375(6585);eabi6983 [Pubmed: 35271311]
Throughput
- High Throughput
Additional Notes
- Bait generated from library of CRISPR-edited human embryonic kidney (HEK) 293T cell lines harboring fluorescent tags on individual proteins
Curated By
- BioGRID