BAIT
CANX
CNX, IP90, P90
calnexin
GO Process (9)
GO Function (2)
GO Component (7)
Gene Ontology Biological Process
- antigen processing and presentation of exogenous peptide antigen via MHC class II [TAS]
- antigen processing and presentation of peptide antigen via MHC class I [TAS]
- cellular protein metabolic process [TAS]
- clathrin-mediated endocytosis [ISS]
- post-translational protein modification [TAS]
- protein N-linked glycosylation via asparagine [TAS]
- protein folding [TAS]
- protein secretion [TAS]
- synaptic vesicle endocytosis [ISS]
Gene Ontology Molecular Function
Homo sapiens
PREY
ENPP1
ARHR2, COLED, M6S1, NPP1, NPPS, PC-1, PCA1, PDNP1
ectonucleotide pyrophosphatase/phosphodiesterase 1
GO Process (21)
GO Function (10)
GO Component (7)
Gene Ontology Biological Process
- 3'-phosphoadenosine 5'-phosphosulfate metabolic process [IDA]
- ATP catabolic process [ISS]
- cellular phosphate ion homeostasis [IDA]
- cellular response to insulin stimulus [IDA]
- generation of precursor metabolites and energy [IDA]
- inorganic diphosphate transport [IDA]
- negative regulation of cell growth [IDA]
- negative regulation of fat cell differentiation [IDA]
- negative regulation of glucose import [IDA]
- negative regulation of glycogen biosynthetic process [IDA]
- negative regulation of insulin receptor signaling pathway [IDA]
- negative regulation of protein autophosphorylation [IDA]
- nucleic acid phosphodiester bond hydrolysis [ISS]
- nucleoside triphosphate catabolic process [IDA]
- phosphate-containing compound metabolic process [IDA]
- regulation of bone mineralization [IC]
- riboflavin metabolic process [TAS]
- sequestering of triglyceride [IDA]
- small molecule metabolic process [TAS]
- vitamin metabolic process [TAS]
- water-soluble vitamin metabolic process [TAS]
Gene Ontology Molecular Function- 3'-phosphoadenosine 5'-phosphosulfate binding [IC]
- ATP binding [IDA]
- calcium ion binding [ISS]
- insulin receptor binding [IDA]
- nucleoside-triphosphate diphosphatase activity [IDA]
- nucleotide diphosphatase activity [IDA, TAS]
- phosphodiesterase I activity [ISS]
- protein binding [IPI]
- protein homodimerization activity [IDA]
- zinc ion binding [ISS]
- 3'-phosphoadenosine 5'-phosphosulfate binding [IC]
- ATP binding [IDA]
- calcium ion binding [ISS]
- insulin receptor binding [IDA]
- nucleoside-triphosphate diphosphatase activity [IDA]
- nucleotide diphosphatase activity [IDA, TAS]
- phosphodiesterase I activity [ISS]
- protein binding [IPI]
- protein homodimerization activity [IDA]
- zinc ion binding [ISS]
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
OpenCell: Endogenous tagging for the cartography of human cellular organization.
Elucidating the wiring diagram of the human cell is a central goal of the postgenomic era. We combined genome engineering, confocal live-cell imaging, mass spectrometry, and data science to systematically map the localization and interactions of human proteins. Our approach provides a data-driven description of the molecular and spatial networks that organize the proteome. Unsupervised clustering of these networks delineates ... [more]
Science Dec. 11, 2021; 375(6585);eabi6983 [Pubmed: 35271311]
Throughput
- High Throughput
Additional Notes
- Bait generated from library of CRISPR-edited human embryonic kidney (HEK) 293T cell lines harboring fluorescent tags on individual proteins
Curated By
- BioGRID