CANX
Gene Ontology Biological Process
- antigen processing and presentation of exogenous peptide antigen via MHC class II [TAS]
- antigen processing and presentation of peptide antigen via MHC class I [TAS]
- cellular protein metabolic process [TAS]
- clathrin-mediated endocytosis [ISS]
- post-translational protein modification [TAS]
- protein N-linked glycosylation via asparagine [TAS]
- protein folding [TAS]
- protein secretion [TAS]
- synaptic vesicle endocytosis [ISS]
Gene Ontology Molecular Function
TOR1A
Gene Ontology Biological Process
- ATP catabolic process [IDA]
- ER-associated misfolded protein catabolic process [ISS]
- cell adhesion [IMP]
- chaperone-mediated protein folding [IDA]
- chaperone-mediated protein transport [IDA]
- intermediate filament cytoskeleton organization [IMP]
- neuron projection development [IMP]
- nuclear envelope organization [ISS]
- nuclear membrane organization [ISS]
- organelle organization [ISS]
- positive regulation of synaptic vesicle endocytosis [IMP]
- protein deneddylation [IMP]
- protein homooligomerization [IDA]
- protein localization to nucleus [IMP, ISS]
- regulation of dopamine uptake involved in synaptic transmission [IDA]
- regulation of protein localization to cell surface [IMP]
- synaptic vesicle transport [IMP]
- wound healing, spreading of cells [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- cytoplasmic vesicle membrane [ISS]
- endoplasmic reticulum lumen [IDA]
- extracellular vesicular exosome [IDA]
- extrinsic component of endoplasmic reticulum membrane [IDA]
- growth cone [ISS]
- intracellular membrane-bounded organelle [IDA]
- membrane [IDA]
- nuclear envelope [ISS]
- nuclear membrane [IDA]
- secretory granule [ISS]
- synaptic vesicle [IDA]
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
OpenCell: Endogenous tagging for the cartography of human cellular organization.
Elucidating the wiring diagram of the human cell is a central goal of the postgenomic era. We combined genome engineering, confocal live-cell imaging, mass spectrometry, and data science to systematically map the localization and interactions of human proteins. Our approach provides a data-driven description of the molecular and spatial networks that organize the proteome. Unsupervised clustering of these networks delineates ... [more]
Throughput
- High Throughput
Additional Notes
- Bait generated from library of CRISPR-edited human embryonic kidney (HEK) 293T cell lines harboring fluorescent tags on individual proteins
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| TOR1A CANX | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.7698 | BioGRID | 1182817 | |
| TOR1A CANX | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.9507 | BioGRID | 3120391 | |
| TOR1A CANX | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | - | |
| TOR1A CANX | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID