BAIT

KAT3

A. thaliana low-K+-tolerant 1, ARABIDOPSIS THALIANA K+ RECTIFYING CHANNEL 1, ATKC1, AtLKT1, F4D11.150, F4D11_150, K+ INWARD RECTIFYING CHANNEL PROTEIN, KC1, potassium channel in Arabidopsis thaliana 3, AT4G32650
potassium channel KAT3
GO Process (2)
GO Function (3)
GO Component (3)
Arabidopsis thaliana (Columbia)
PREY

KT1

AKT1, ATAKT1, F18A8.2, F18A8_2, K+ transporter 1, POTASSIUM TRANSPORTER, AT2G26650
potassium channel AKT1
Arabidopsis thaliana (Columbia)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Regulated expression of Arabidopsis shaker K+ channel genes involved in K+ uptake and distribution in the plant.

Pilot G, Gaymard F, Mouline K, Cherel I, Sentenac H

Potassium is the most abundant cation in the cytosol, where it plays a role in basal functions. Rapid uptake and distribution of K+ is therefore required for plant growth. Three members of the so-called Shaker K+ channel gene family (nine genes identified in Arabidopsis) play a role in these transports: AKT1, SKOR and AKT2. The encoded proteins are involved in ... [more]

Plant Mol. Biol. Mar. 01, 2003; 51(5);773-87 [Pubmed: 12678562]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
KAT3 KT1
PCA
PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Low-BioGRID
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Curated By

  • BioGRID