BAIT

KAT3

A. thaliana low-K+-tolerant 1, ARABIDOPSIS THALIANA K+ RECTIFYING CHANNEL 1, ATKC1, AtLKT1, F4D11.150, F4D11_150, K+ INWARD RECTIFYING CHANNEL PROTEIN, KC1, potassium channel in Arabidopsis thaliana 3, AT4G32650
potassium channel KAT3
GO Process (2)
GO Function (3)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

KT1

AKT1, ATAKT1, F18A8.2, F18A8_2, K+ transporter 1, POTASSIUM TRANSPORTER, AT2G26650
potassium channel AKT1
Arabidopsis thaliana (Columbia)

PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

Heteromeric AtKC1{middle dot}AKT1 channels in Arabidopsis roots facilitate growth under K+-limiting conditions.

Geiger D, Becker D, Vosloh D, Gambale F, Palme K, Rehers M, Anschuetz U, Dreyer I, Kudla J, Hedrich R

Plant growth and development is driven by osmotic processes. Potassium represents the major osmotically active cation in plants cells. The uptake of this inorganic osmolyte from the soil in Arabidopsis involves a root K(+) uptake module consisting of the two K(+) channel alpha-subunits, AKT1 and AtKC1. AKT1-mediated potassium absorption from K(+)-depleted soil was shown to depend on the calcium-sensing proteins ... [more]

J. Biol. Chem. Aug. 07, 2009; 284(32);21288-95 [Pubmed: 19509299]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
KAT3 KT1
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID