BAIT
GOSR1
GOLIM2, GOS-28, GOS28, GOS28/P28, GS28, P28
golgi SNAP receptor complex member 1
GO Process (2)
GO Function (1)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
ZW10
HZW10, KNTC1AP
zw10 kinetochore protein
GO Process (11)
GO Function (2)
GO Component (8)
Gene Ontology Biological Process
- ER to Golgi vesicle-mediated transport [IMP]
- Golgi organization [IGI, IMP]
- establishment of mitotic spindle orientation [IMP]
- meiotic nuclear division [TAS]
- mitotic cell cycle [TAS]
- mitotic cell cycle checkpoint [IDA]
- mitotic metaphase plate congression [IMP]
- mitotic sister chromatid segregation [IDA]
- protein complex assembly [IDA]
- protein localization to kinetochore [IMP]
- regulation of exit from mitosis [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
OpenCell: Endogenous tagging for the cartography of human cellular organization.
Elucidating the wiring diagram of the human cell is a central goal of the postgenomic era. We combined genome engineering, confocal live-cell imaging, mass spectrometry, and data science to systematically map the localization and interactions of human proteins. Our approach provides a data-driven description of the molecular and spatial networks that organize the proteome. Unsupervised clustering of these networks delineates ... [more]
Science Dec. 11, 2021; 375(6585);eabi6983 [Pubmed: 35271311]
Throughput
- High Throughput
Additional Notes
- Bait generated from library of CRISPR-edited human embryonic kidney (HEK) 293T cell lines harboring fluorescent tags on individual proteins
Curated By
- BioGRID