BAIT
IPO5
IMB3, KPNB3, Pse1, RANBP5, imp5, RP11-72J7.1
importin 5
GO Process (5)
GO Function (4)
GO Component (8)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
CENPE
CENP-E, KIF10, MCPH13, PPP1R61
centromere protein E, 312kDa
GO Process (12)
GO Function (4)
GO Component (14)
Gene Ontology Biological Process
- ATP catabolic process [IBA]
- antigen processing and presentation of exogenous peptide antigen via MHC class II [TAS]
- attachment of mitotic spindle microtubules to kinetochore [IMP]
- blood coagulation [TAS]
- kinetochore assembly [NAS]
- metabolic process [IMP]
- microtubule-based movement [IBA, TAS]
- mitotic cell cycle [IBA, TAS]
- mitotic chromosome movement towards spindle pole [TAS]
- mitotic metaphase plate congression [IMP]
- positive regulation of protein kinase activity [IMP]
- regulation of mitotic metaphase/anaphase transition [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- chromosome [IDA]
- chromosome, centromeric region [IDA]
- condensed chromosome outer kinetochore [TAS]
- condensed chromosome, centromeric region [IDA]
- cytoplasm [IDA]
- cytosol [TAS]
- kinesin complex [IBA]
- kinetochore [IDA]
- membrane [IDA]
- microtubule [IDA]
- microtubule cytoskeleton [IDA]
- midbody [IDA]
- mitotic spindle midzone [IDA]
- nucleus [IMP]
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
OpenCell: Endogenous tagging for the cartography of human cellular organization.
Elucidating the wiring diagram of the human cell is a central goal of the postgenomic era. We combined genome engineering, confocal live-cell imaging, mass spectrometry, and data science to systematically map the localization and interactions of human proteins. Our approach provides a data-driven description of the molecular and spatial networks that organize the proteome. Unsupervised clustering of these networks delineates ... [more]
Science Dec. 11, 2021; 375(6585);eabi6983 [Pubmed: 35271311]
Throughput
- High Throughput
Additional Notes
- Bait generated from library of CRISPR-edited human embryonic kidney (HEK) 293T cell lines harboring fluorescent tags on individual proteins
Curated By
- BioGRID