BAIT
PLA2G4A
PLA2G4, cPLA2-alpha
phospholipase A2, group IVA (cytosolic, calcium-dependent)
GO Process (15)
GO Function (4)
GO Component (4)
Gene Ontology Biological Process
- arachidonic acid metabolic process [TAS]
- blood coagulation [TAS]
- cardiolipin acyl-chain remodeling [TAS]
- glycerophospholipid biosynthetic process [TAS]
- icosanoid metabolic process [NAS]
- phosphatidic acid biosynthetic process [TAS]
- phosphatidylcholine acyl-chain remodeling [TAS]
- phosphatidylethanolamine acyl-chain remodeling [TAS]
- phosphatidylglycerol acyl-chain remodeling [TAS]
- phosphatidylinositol acyl-chain remodeling [TAS]
- phosphatidylserine acyl-chain remodeling [TAS]
- phospholipid metabolic process [TAS]
- platelet activating factor biosynthetic process [NAS]
- platelet activation [TAS]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
DNAJC17
DnaJ (Hsp40) homolog, subfamily C, member 17
GO Process (0)
GO Function (0)
GO Component (0)
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
OpenCell: Endogenous tagging for the cartography of human cellular organization.
Elucidating the wiring diagram of the human cell is a central goal of the postgenomic era. We combined genome engineering, confocal live-cell imaging, mass spectrometry, and data science to systematically map the localization and interactions of human proteins. Our approach provides a data-driven description of the molecular and spatial networks that organize the proteome. Unsupervised clustering of these networks delineates ... [more]
Science Dec. 11, 2021; 375(6585);eabi6983 [Pubmed: 35271311]
Throughput
- High Throughput
Additional Notes
- Bait generated from library of CRISPR-edited human embryonic kidney (HEK) 293T cell lines harboring fluorescent tags on individual proteins
Curated By
- BioGRID