BAIT
PRPF19
NMP200, PRP19, PSO4, SNEV, UBOX4, hPSO4
pre-mRNA processing factor 19
GO Process (10)
GO Function (4)
GO Component (10)
Gene Ontology Biological Process
- cellular protein localization [IMP]
- double-strand break repair via nonhomologous end joining [IMP]
- generation of catalytic spliceosome for first transesterification step [IBA]
- mRNA splicing, via spliceosome [IC, IDA]
- proteasomal protein catabolic process [IMP]
- protein K63-linked ubiquitination [IMP]
- protein polyubiquitination [IDA]
- signal transduction involved in DNA damage checkpoint [IMP]
- spliceosomal complex assembly [IMP]
- spliceosomal tri-snRNP complex assembly [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
ACLY
ACL, ATPCL, CLATP
ATP citrate lyase
GO Process (10)
GO Function (2)
GO Component (7)
Gene Ontology Biological Process
- ATP catabolic process [TAS]
- cellular lipid metabolic process [TAS]
- citrate metabolic process [TAS]
- coenzyme A metabolic process [TAS]
- energy reserve metabolic process [TAS]
- lipid biosynthetic process [IDA]
- long-chain fatty-acyl-CoA biosynthetic process [TAS]
- positive regulation of cellular metabolic process [TAS]
- small molecule metabolic process [TAS]
- triglyceride biosynthetic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
OpenCell: Endogenous tagging for the cartography of human cellular organization.
Elucidating the wiring diagram of the human cell is a central goal of the postgenomic era. We combined genome engineering, confocal live-cell imaging, mass spectrometry, and data science to systematically map the localization and interactions of human proteins. Our approach provides a data-driven description of the molecular and spatial networks that organize the proteome. Unsupervised clustering of these networks delineates ... [more]
Science Dec. 11, 2021; 375(6585);eabi6983 [Pubmed: 35271311]
Throughput
- High Throughput
Additional Notes
- Bait generated from library of CRISPR-edited human embryonic kidney (HEK) 293T cell lines harboring fluorescent tags on individual proteins
Curated By
- BioGRID