BAIT
SLC12A2
BSC, BSC2, NKCC1, PPP1R141
solute carrier family 12 (sodium/potassium/chloride transporter), member 2
GO Process (8)
GO Function (2)
GO Component (5)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
SRSF1
ASF, SF2, SF2p33, SFRS1, SRp30a, OK/SW-cl.3
serine/arginine-rich splicing factor 1
GO Process (10)
GO Function (4)
GO Component (6)
Gene Ontology Biological Process
- RNA splicing [TAS]
- gene expression [TAS]
- mRNA 3'-end processing [TAS]
- mRNA 5'-splice site recognition [IDA]
- mRNA export from nucleus [TAS]
- mRNA processing [TAS]
- mRNA splice site selection [TAS]
- mRNA splicing, via spliceosome [IC, TAS]
- termination of RNA polymerase II transcription [TAS]
- transcription from RNA polymerase II promoter [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
OpenCell: Endogenous tagging for the cartography of human cellular organization.
Elucidating the wiring diagram of the human cell is a central goal of the postgenomic era. We combined genome engineering, confocal live-cell imaging, mass spectrometry, and data science to systematically map the localization and interactions of human proteins. Our approach provides a data-driven description of the molecular and spatial networks that organize the proteome. Unsupervised clustering of these networks delineates ... [more]
Science Dec. 11, 2021; 375(6585);eabi6983 [Pubmed: 35271311]
Throughput
- High Throughput
Additional Notes
- Bait generated from library of CRISPR-edited human embryonic kidney (HEK) 293T cell lines harboring fluorescent tags on individual proteins
Curated By
- BioGRID