Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

SNX27-driven membrane localisation of OTULIN antagonises linear ubiquitination and NF-?B signalling activation.

Shi R, Shi X, Qin D, Tang S, Vermeulen M, Zhang X

Linear ubiquitination is a novel type of ubiquitination that plays important physiological roles in signalling pathways such as tumour necrosis factor (TNF) signalling. However, little is known about the regulatory mechanisms of linear ubiquitination, except the well-described enzymatic regulators E3 ligase linear ubiquitin chain assembly complex (LUBAC) and deubiquitinase OTULIN.Previously, we identified SNX27, a member of the sorting nexin family ... [more]

Cell Biosci Jul. 27, 2021; 11(1);146 [Pubmed: 34315543]

Throughput

  • High Throughput

Additional Notes

  • Affinity Capture-MS was carried out to identify high confidence protein-protein interactors with a fold change of >2 over control and an adjusted p<0.05

Curated By

  • BioGRID