Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Interactome analysis of Bag-1 isoforms reveals novel interaction partners in endoplasmic reticulum-associated degradation.

Can ND, Basturk E, Kizilboga T, Akcay IM, Dingiloglu B, Tatli O, Acar S, Ozfiliz Kilbas P, Elbeyli E, Muratcioglu S, Jannuzzi AT, Gursoy A, Keskin O, Doganay HL, Karademir Yilmaz B, Dinler Doganay G

Bag-1 is a multifunctional protein that regulates Hsp70 chaperone activity, apoptosis, and proliferation. The three major Bag-1 isoforms have different subcellular localizations and partly non-overlapping functions. To identify the detailed interaction network of each isoform, we utilized mass spectrometry-based proteomics and found that interactomes of Bag-1 isoforms contained many common proteins, with variations in their abundances. Bag-1 interactomes were enriched ... [more]

PLoS One Aug. 25, 2021; 16(8);e0256640 [Pubmed: 34428256]

Quantitative Score

  • 1.26 [FoldChange]

Throughput

  • High Throughput

Additional Notes

  • Affinity Capture-MS was carried out to identify high confidence protein-protein interactors with a >1.2 fold enrichment over control (Fold enrichment reported here)
  • Bag-1S Fold Change: 1.26

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
BAG1 ACTR2
Co-fractionation
Co-fractionation

Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

High-BioGRID
919914

Curated By

  • BioGRID