BAIT
CLPP
DFNB81, PRLTS3
caseinolytic mitochondrial matrix peptidase proteolytic subunit
GO Process (1)
GO Function (3)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
ACSL3
ACS3, FACL3, PRO2194
acyl-CoA synthetase long-chain family member 3
GO Process (6)
GO Function (3)
GO Component (5)
Gene Ontology Biological Process
- long-chain fatty acid import [IDA]
- long-chain fatty acid metabolic process [IMP]
- positive regulation of Golgi to plasma membrane protein transport [IMP]
- positive regulation of phosphatidylcholine biosynthetic process [IMP]
- positive regulation of secretion [IMP]
- very-low-density lipoprotein particle assembly [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
Variability in Streptavidin-Sepharose Matrix Quality Can Significantly Affect Proximity-Dependent Biotinylation (BioID) Data.
Due to their ease of use and high binding affinity, streptavidin-based purification tools have become widely used for isolating biotinylated compounds from complex mixtures. We and others routinely use streptavidin-sepharose matrices to isolate biotinylated polypeptides generated in proximity-dependent biotinylation approaches, such as BioID or APEX. However, we noted sporadic, substantial variation in the quality of BioID experiments performed in the ... [more]
J Proteome Res Dec. 07, 2019; 19(8);3554-3561 [Pubmed: 32628020]
Throughput
- High Throughput
Additional Notes
- BFDR less or equal to 0.01
- BioID
Curated By
- BioGRID