An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

PROTAC-mediated degradation reveals a non-catalytic function of AURORA-A kinase.

Adhikari B, Bozilovic J, Diebold M, Schwarz JD, Hofstetter J, Schroeder M, Wanior M, Narain A, Vogt M, Dudvarski Stankovic N, Baluapuri A, Schoenemann L, Eing L, Bhandare P, Kuster B, Schlosser A, Heinzlmeir S, Sotriffer C, Knapp S, Wolf E

The mitotic kinase AURORA-A is essential for cell cycle progression and is considered a priority cancer target. Although the catalytic activity of AURORA-A is essential for its mitotic function, recent reports indicate an additional non-catalytic function, which is difficult to target by conventional small molecules. We therefore developed a series of chemical degraders (PROTACs) by connecting a clinical kinase inhibitor ... [more]

Nat Chem Biol Dec. 01, 2019; 16(11);1179-1188 [Pubmed: 32989298]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

AURKA

Gene Ontology Biological Process

Gene Ontology Molecular Function

histone serine kinase activity [IBA]protein binding [IPI]protein kinase activity [IDA]protein kinase binding [IPI]protein serine/threonine kinase activity [EXP]protein serine/threonine/tyrosine kinase activity [TAS]

Gene Ontology Cellular Component

CRBN