BAIT

GLE1

BRR3, NLE2, RSS1, nucleoporin GLE1, L000003399, L000001255, S000029324, YDL207W
Cytoplasmic nucleoporin required for polyadenylated mRNA export; contains a nuclear export signal; when bound to inositol hexakisphosphate (IP6), functions as an activator for the Dbp5p ATPase activity at the nuclear pore complex during mRNA export; mediates translation initiation; required for efficient translation termination
Saccharomyces cerevisiae (S288c)
PREY

NSP1

FG-nucleoporin NSP1, L000001277, YJL041W
FG-nucleoporin component of central core of the nuclear pore complex; also part of the nuclear pore complex (NPC) nuclear basket; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nup82p, Gle2p and two other FG-nucleoporins (Nup159p and Nup116p); also found in stable complex with Nic96p and two other FG-nucleoproteins (Nup49p and Nup57p)
Saccharomyces cerevisiae (S288c)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Maturation Kinetics of a Multiprotein Complex Revealed by Metabolic Labeling.

Onischenko E, Noor E, Fischer JS, Gillet L, Wojtynek M, Vallotton P, Weis K

All proteins interact with other cellular components to fulfill their function. While tremendous progress has been made in the identification of protein complexes, their assembly and dynamics remain difficult to characterize. Here, we present a high-throughput strategy to analyze the native assembly kinetics of protein complexes. We apply our approach to characterize the co-assembly for 320 pairs of nucleoporins (NUPs) ... [more]

Cell Dec. 23, 2019; 183(7);1785-1800.e26 [Pubmed: 33333025]

Throughput

  • High Throughput

Additional Notes

  • KARMA

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
GLE1 NSP1
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Low-BioGRID
-
NSP1 GLE1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.1927BioGRID
1938860

Curated By

  • BioGRID