BAIT

SPT4

transcription elongation factor SPT4, L000002030, YGR063C

Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions

GO Process (12)

GO Function (3)

GO Component (5)

Gene Ontology Molecular Function

core RNA polymerase I binding transcription factor activity [IC]
core RNA polymerase II binding transcription factor activity [IC]
rDNA binding [IDA]

Gene Ontology Cellular Component

DSIF complex [IPI]

kinetochore [IDA]

mating-type region heterochromatin [IDA]

nuclear telomeric heterochromatin [IDA]

rDNA heterochromatin [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

SPT5

transcription elongation factor SPT5, L000002031, YML010W

GO Process (8)

GO Function (7)

GO Component (4)

Gene Ontology Biological Process

7-methylguanosine mRNA capping [IGI, IPI]

intracellular mRNA localization [IMP]

mRNA splicing, via spliceosome [IMP]

negative regulation of transcription elongation from RNA polymerase I promoter [IGI]

positive regulation of transcription elongation from RNA polymerase I promoter [IMP]

positive regulation of transcription elongation from RNA polymerase II promoter [IMP]

regulation of rRNA processing [IMP]

regulation of transcription-coupled nucleotide-excision repair [IGI]

Gene Ontology Molecular Function

RNA polymerase I core binding [IPI]
RNA polymerase I transcription factor binding [IPI]
RNA polymerase II core binding [IPI]
core RNA polymerase I binding transcription factor activity [IC]
core RNA polymerase II binding transcription factor activity [IGI, IMP, IPI]
mRNA binding [IDA]
rRNA binding [IDA]

Gene Ontology Cellular Component

DSIF complex [IPI]

mitochondrion [IDA]

nucleus [IDA]

rDNA heterochromatin [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Co-crystal Structure

Interaction directly demonstrated at the atomic level by X-ray crystallography. Also used for NMR or Electron Microscopy (EM) structures. If there is no obvious bait-hit directionality to the interaction involving 3 or more proteins, then the co-crystallized proteins should be listed as a complex.

Publication

Core structure of the yeast spt4-spt5 complex: a conserved module for regulation of transcription elongation.

Guo M, Xu F, Yamada J, Egelhofer T, Gao Y, Hartzog GA, Teng M, Niu L

The Spt4-Spt5 complex is an essential RNA polymerase II elongation factor found in all eukaryotes and important for gene regulation. We report here the crystal structure of Saccharomyces cerevisiae Spt4 bound to the NGN domain of Spt5. This structure reveals that Spt4-Spt5 binding is governed by an acid-dipole interaction between Spt5 and Spt4. Mutations that disrupt this interaction disrupt the ... [more]

Structure Nov. 12, 2008; 16(11);1649-58 [Pubmed: 19000817]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
SPT5 SPT4	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Gavin AC (2006)	High	-	BioGRID	-
SPT5 SPT4	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Gingras AC (2005)	Low	-	BioGRID	-
SPT4 SPT5	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Krogan NJ (2002)	Low	-	BioGRID	-
SPT5 SPT4	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Krogan NJ (2002)	Low	-	BioGRID	-
SPT4 SPT5	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Krogan NJ (2006)	High	-	BioGRID	-
SPT4 SPT5	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Lindstrom DL (2003)	High	-	BioGRID	-
SPT5 SPT4	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Lindstrom DL (2003)	High	-	BioGRID	-
SPT4 SPT5	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Michaelis AC (2023)	High	10	BioGRID	3597471
SPT4 SPT5	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Mosley AL (2013)	Low	-	BioGRID	-
SPT5 SPT4	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Guo M (2008)	Low	-	BioGRID	-
SPT4 SPT5	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Hartzog GA (1998)	Low	-	BioGRID	-
SPT5 SPT4	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Hartzog GA (1998)	Low	-	BioGRID	-
SPT4 SPT5	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Silva A (2017)	Low	-	BioGRID	-
SPT5 SPT4	Co-purification Co-purification An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps.	Crickard JB (2016)	Low	-	BioGRID	-
SPT4 SPT5	Dosage Rescue Dosage Rescue A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.	Ding B (2010)	Low	-	BioGRID	353986
SPT4 SPT5	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Blythe A (2014)	Low	-	BioGRID	-
SPT4 SPT5	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Blythe AJ (2016)	Low	-	BioGRID	-
SPT4 SPT5	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Martinez-Rucobo FW (2011)	Low	-	BioGRID	-
SPT4 SPT5	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Anderson SJ (2011)	Low	-	BioGRID	559095
SPT4 SPT5	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Swanson MS (1992)	Low	-	BioGRID	158928

Curated By

BioGRID

Interaction Summary

SPT4

Gene Ontology Biological Process

Gene Ontology Molecular Function

core RNA polymerase I binding transcription factor activity [IC]core RNA polymerase II binding transcription factor activity [IC]rDNA binding [IDA]

Gene Ontology Cellular Component

SPT5

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase I core binding [IPI]RNA polymerase I transcription factor binding [IPI]RNA polymerase II core binding [IPI]core RNA polymerase I binding transcription factor activity [IC]core RNA polymerase II binding transcription factor activity [IGI, IMP, IPI]mRNA binding [IDA]rRNA binding [IDA]

Gene Ontology Cellular Component

Co-crystal Structure

Publication

Core structure of the yeast spt4-spt5 complex: a conserved module for regulation of transcription elongation.

Throughput

Related interactions

Curated By

core RNA polymerase I binding transcription factor activity [IC]
core RNA polymerase II binding transcription factor activity [IC]
rDNA binding [IDA]