BAIT
RLIM
NY-REN-43, RNF12, CTD-2530H13.3
ring finger protein, LIM domain interacting
GO Process (4)
GO Function (2)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
AKT1
AKT, CWS6, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
v-akt murine thymoma viral oncogene homolog 1
GO Process (73)
GO Function (12)
GO Component (6)
Gene Ontology Biological Process
- Fc-epsilon receptor signaling pathway [TAS]
- G-protein coupled receptor signaling pathway [TAS]
- RNA metabolic process [TAS]
- T cell costimulation [TAS]
- activation-induced cell death of T cells [IMP]
- apoptotic process [TAS]
- blood coagulation [TAS]
- cell differentiation [TAS]
- cell proliferation [TAS]
- cellular protein modification process [TAS]
- cellular response to insulin stimulus [IMP, ISS]
- endocrine pancreas development [TAS]
- epidermal growth factor receptor signaling pathway [TAS]
- fibroblast growth factor receptor signaling pathway [TAS]
- gene expression [TAS]
- innate immune response [TAS]
- insulin receptor signaling pathway [IMP]
- insulin-like growth factor receptor signaling pathway [IMP]
- intracellular signal transduction [IDA]
- intrinsic apoptotic signaling pathway [TAS]
- mRNA metabolic process [TAS]
- mammary gland epithelial cell differentiation [TAS]
- membrane organization [TAS]
- negative regulation of apoptotic process [IDA]
- negative regulation of autophagy [IMP]
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process [ISS]
- negative regulation of endopeptidase activity [IMP]
- negative regulation of extrinsic apoptotic signaling pathway in absence of ligand [TAS]
- negative regulation of fatty acid beta-oxidation [IMP]
- negative regulation of neuron death [NAS]
- negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway [NAS]
- negative regulation of plasma membrane long-chain fatty acid transport [IMP]
- negative regulation of protein kinase activity [IMP, ISS]
- negative regulation of proteolysis [IMP]
- negative regulation of release of cytochrome c from mitochondria [ISS]
- neurotrophin TRK receptor signaling pathway [TAS]
- nitric oxide biosynthetic process [TAS]
- nitric oxide metabolic process [TAS]
- peptidyl-serine phosphorylation [IDA]
- phosphatidylinositol-mediated signaling [TAS]
- phosphorylation [IDA]
- platelet activation [TAS]
- positive regulation of blood vessel endothelial cell migration [IDA]
- positive regulation of cell growth [IDA]
- positive regulation of cellular protein metabolic process [ISS]
- positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cycle [IDA]
- positive regulation of endothelial cell proliferation [IMP]
- positive regulation of establishment of protein localization to plasma membrane [IMP]
- positive regulation of fat cell differentiation [IMP]
- positive regulation of glucose import [IMP]
- positive regulation of glucose metabolic process [IMP]
- positive regulation of glycogen biosynthetic process [IMP, NAS]
- positive regulation of lipid biosynthetic process [IMP]
- positive regulation of nitric oxide biosynthetic process [IMP]
- positive regulation of nitric-oxide synthase activity [IMP]
- positive regulation of peptidyl-serine phosphorylation [IDA]
- positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway [TAS]
- positive regulation of protein phosphorylation [IDA]
- positive regulation of sequence-specific DNA binding transcription factor activity [IDA]
- protein autophosphorylation [TAS]
- protein import into nucleus, translocation [IMP]
- protein phosphorylation [IDA]
- regulation of cell cycle checkpoint [TAS]
- regulation of cell migration [IMP, TAS]
- regulation of glycogen biosynthetic process [IMP]
- regulation of neuron projection development [ISS]
- regulation of nitric-oxide synthase activity [TAS]
- response to UV-A [IDA]
- response to fluid shear stress [IMP]
- response to heat [TAS]
- response to oxidative stress [ISS]
- signal transduction [TAS]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function- 14-3-3 protein binding [IPI]
- ATP binding [IC, IDA]
- enzyme binding [ISS]
- identical protein binding [IPI]
- kinase activity [IDA]
- nitric-oxide synthase regulator activity [IMP]
- phosphatidylinositol-3,4,5-trisphosphate binding [IDA]
- phosphatidylinositol-3,4-bisphosphate binding [IDA]
- protein binding [IPI]
- protein kinase activity [TAS]
- protein serine/threonine kinase activity [IDA, TAS]
- protein serine/threonine/tyrosine kinase activity [IDA]
- 14-3-3 protein binding [IPI]
- ATP binding [IC, IDA]
- enzyme binding [ISS]
- identical protein binding [IPI]
- kinase activity [IDA]
- nitric-oxide synthase regulator activity [IMP]
- phosphatidylinositol-3,4,5-trisphosphate binding [IDA]
- phosphatidylinositol-3,4-bisphosphate binding [IDA]
- protein binding [IPI]
- protein kinase activity [TAS]
- protein serine/threonine kinase activity [IDA, TAS]
- protein serine/threonine/tyrosine kinase activity [IDA]
Homo sapiens
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
RNF12 is regulated by AKT phosphorylation and promotes TGF-? driven breast cancer metastasis.
Transforming growth factor-? (TGF-?) acts as a pro-metastatic factor in advanced breast cancer. RNF12, an E3 ubiquitin ligase, stimulates TGF-? signaling by binding to the inhibitory SMAD7 and inducing its proteasomal degradation. How RNF12 activity is regulated and its exact role in cancer is incompletely understood. Here we report that RNF12 was overexpressed in invasive breast cancers and its high ... [more]
Cell Death Dis Dec. 10, 2021; 13(1);44 [Pubmed: 35013159]
Throughput
- Low Throughput
Curated By
- BioGRID