BAIT

ASH1

DNA-binding transcription repressor ASH1, L000003058, YKL185W
Component of the Rpd3L histone deacetylase complex; zinc-finger inhibitor of HO transcription; mRNA is localized and translated in the distal tip of anaphase cells, resulting in accumulation of Ash1p in daughter cell nuclei and inhibition of HO expression; potential Cdc28p substrate
Saccharomyces cerevisiae (S288c)
PREY

TUP1

AAR1, AER2, AMM1, CRT4, CYC9, FLK1, ROX4, SFL2, UMR7, chromatin-silencing transcriptional regulator TUP1, L000002391, YCR084C
General repressor of transcription; forms complex with Cyc8p, involved in the establishment of repressive chromatin structure through interactions with histones H3 and H4, appears to enhance expression of some genes
Saccharomyces cerevisiae (S288c)

Phenotypic Suppression

A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

Genetic analysis argues for a coactivator function for the Saccharomyces cerevisiae Tup1 corepressor.

Parnell EJ, Parnell TJ, Stillman DJ

The Tup1-Cyc8 corepressor complex of Saccharomyces cerevisiae is recruited to promoters by DNA-binding proteins to repress transcription of genes, including the a-specific mating-type genes. We report here a tup1(S649F) mutant that displays mating irregularities and an ?-predominant growth defect. RNA-Seq and ChIP-Seq were used to analyze gene expression and Tup1 occupancy changes in mutant vs wild type in both a ... [more]

Genetics Dec. 02, 2020; 219(2); [Pubmed: 34849878]

Throughput

  • Low Throughput

Ontology Terms

  • rna accumulation (APO:0000224)

Additional Notes

  • Figure 3
  • genetic complex
  • multiple tup1 alleles
  • tup1 mutation in swi5 ash1 double mutant increases HO RNA expression

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
ASH1 TUP1
Phenotypic Enhancement
Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Low-BioGRID
2562049

Curated By

  • BioGRID