BAIT

C19ORF35

chromosome 19 open reading frame 35
GO Process (0)
GO Function (0)
GO Component (0)
Homo sapiens
PREY

SHC1

SHC, SHCA, RP11-307C12.1
SHC (Src homology 2 domain containing) transforming protein 1
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Distinct PEAK3 interactors and outputs expand the signaling potential of the PEAK pseudokinase family.

Hou J, Nguyen EV, Surudoi M, Roy MJ, Patel O, Lucet IS, Ma X, Daly RJ

The pseudokinase scaffolds PEAK1 and PEAK2 are implicated in cancer cell migration and metastasis. We characterized the regulation and role of the third family member PEAK3 in cell signaling. Similar to PEAK1 and PEAK2, PEAK3 formed both homotypic and heterotypic complexes. In addition, like PEAK1, it bound to the adaptors Grb2 and CrkII. However, unlike PEAK1 and PEAK2, homodimerized PEAK3 ... [more]

Sci Signal Dec. 22, 2021; 15(722);eabj3554 [Pubmed: 35192416]

Throughput

  • Low Throughput

Curated By

  • BioGRID