CBLC
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
AURKA
Gene Ontology Biological Process
- G2/M transition of mitotic cell cycle [TAS]
- anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process [TAS]
- mitotic cell cycle [TAS]
- mitotic nuclear division [TAS]
- mitotic spindle organization [IBA]
- negative regulation of protein binding [IDA]
- positive regulation of mitosis [TAS]
- protein autophosphorylation [TAS]
- protein phosphorylation [IDA]
- regulation of centrosome cycle [TAS]
- regulation of cytokinesis [IBA]
- regulation of protein stability [IMP]
- spindle stabilization [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- centrosome [IDA, TAS]
- chromosome passenger complex [IBA]
- condensed nuclear chromosome, centromeric region [IBA]
- cytosol [TAS]
- microtubule cytoskeleton [IDA]
- midbody [TAS]
- nucleus [IDA]
- perinuclear region of cytoplasm [IDA]
- spindle [TAS]
- spindle microtubule [IDA]
- spindle midzone [IBA]
- spindle pole centrosome [IDA]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Stabilization of AURKA by the E3 ubiquitin ligase CBLC in lung adenocarcinoma.
CBL family proteins (CBL, CBLB and CBLC in mammals) are E3 ubiquitin ligases of protein tyrosine kinases. CBL mediates the lysosomal degradation of activated EGFR through K63-linked ubiquitination, while CBLC has an oncogenic function by positively regulating EGFR activation through K6 and K11-linked ubiquitination in EGFR mutant lung adenocarcinoma (LAD). Here, we used immunoprecipitation and mass spectrometry to study the ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID