MEC1
Gene Ontology Biological Process
- DNA damage induced protein phosphorylation [IMP]
- DNA recombination [IMP]
- DNA replication [IMP]
- histone phosphorylation [IGI, IMP]
- nucleobase-containing compound metabolic process [IGI]
- positive regulation of DNA-dependent DNA replication [IMP]
- reciprocal meiotic recombination [IMP]
- telomere maintenance [IDA]
- telomere maintenance via recombination [IGI]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
MRC1
Gene Ontology Biological Process
- DNA repair [IGI, IMP]
- DNA replication [IMP]
- DNA replication checkpoint [IGI, IMP, IPI]
- chromatin silencing at silent mating-type cassette [IGI, IMP]
- chromatin silencing at telomere [IGI, IMP]
- intra-S DNA damage checkpoint [IMP]
- maintenance of DNA repeat elements [IMP]
- mitotic sister chromatid cohesion [IGI, IMP]
- regulation of nuclear cell cycle DNA replication [IMP]
- replication fork protection [IGI, IMP, IPI]
- telomere maintenance [IMP]
Gene Ontology Cellular Component
Synthetic Growth Defect
A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.
Publication
A regulatory phosphorylation site on Mec1 controls chromatin occupancy of RNA polymerases during replication stress.
Upon replication stress, budding yeast checkpoint kinase Mec1ATR triggers the downregulation of transcription, thereby reducing the level of RNA polymerase (RNAP) on chromatin to facilitate replication fork progression. Here, we identify a hydroxyurea-induced phosphorylation site on Mec1, Mec1-S1991, that contributes to the eviction of RNAPII and RNAPIII during replication stress. The expression of the non-phosphorylatable mec1-S1991A mutant reduces replication fork ... [more]
Throughput
- Low Throughput
Ontology Terms
- phenotype: vegetative growth (APO:0000106)
- phenotype: resistance to chemicals (APO:0000087)
Additional Notes
- double mutants show increased sensitivity to HU
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
MEC1 MRC1 | Biochemical Activity Biochemical Activity An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation. | Low | - | BioGRID | 613120 | |
MEC1 MRC1 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.6577 | BioGRID | 1961525 | |
MEC1 MRC1 | Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell. | Low | - | BioGRID | 2451259 | |
MEC1 MRC1 | Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell. | High | - | BioGRID | 457651 |
Curated By
- BioGRID