Co-localization

Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.

Publication

Interactomic analysis reveals a homeostatic role for the HIV restriction factor TRIM5? in mitophagy.

Saha B, Salemi M, Williams GL, Oh S, Paffett ML, Phinney B, Mandell MA

The protein TRIM5? has multiple roles in antiretroviral defense, but the mechanisms underlying TRIM5? action are unclear. Here, we employ APEX2-based proteomics to identify TRIM5?-interacting partners. Our proteomics results connect TRIM5 to other proteins with actions in antiviral defense. Additionally, they link TRIM5 to mitophagy, an autophagy-based mode of mitochondrial quality control that is compromised in several human diseases. We ... [more]

Cell Rep May. 10, 2022; 39(6);110797 [Pubmed: 35545034]

Throughput

  • Low Throughput

Additional Notes

  • Proximity Ligation Assay

Curated By

  • BioGRID