BAIT
PTPRS
PTPSIGMA
protein tyrosine phosphatase, receptor type, S
GO Process (2)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
ASPM
ASP, Calmbp1, MCPH5, RP11-32D17.1
asp (abnormal spindle) homolog, microcephaly associated (Drosophila)
GO Process (0)
GO Function (0)
GO Component (0)
Homo sapiens
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Combinatorial CRISPR screen identifies fitness effects of gene paralogues.
Genetic redundancy has evolved as a way for human cells to survive the loss of genes that are single copy and essential in other organisms, but also allows tumours to survive despite having highly rearranged genomes. In this study we CRISPR screen 1191 gene pairs, including paralogues and known and predicted synthetic lethal interactions to identify 105 gene combinations whose ... [more]
Nat Commun Dec. 26, 2020; 12(1);1302 [Pubmed: 33637726]
Throughput
- Low Throughput
Ontology Terms
- phenotype: growth abnormality (HP:0001507) [viability (PATO:0000169)]
Additional Notes
- CRISPR GI screen
- Cell Line: A-375 cell BTO:0002806
- Experimental Setup: Timecourse
- GIST: A-phenotypic negative genetic interaction
- Library: Targeted CRISPR synthetic lethality library
- Significance Threshold:FDR<0.1 and additional filtering of gene pairs with one gene with a large individual fitness defect
Curated By
- BioGRID