Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

Combinatorial CRISPR screen identifies fitness effects of gene paralogues.

Thompson NA, Ranzani M, van der Weyden L, Iyer V, Offord V, Droop A, Behan F, Goncalves E, Speak A, Iorio F, Hewinson J, Harle V, Robertson H, Anderson E, Fu B, Yang F, Zagnoli-Vieira G, Chapman P, Del Castillo Velasco-Herrera M, Garnett MJ, Jackson SP, Adams DJ

Genetic redundancy has evolved as a way for human cells to survive the loss of genes that are single copy and essential in other organisms, but also allows tumours to survive despite having highly rearranged genomes. In this study we CRISPR screen 1191 gene pairs, including paralogues and known and predicted synthetic lethal interactions to identify 105 gene combinations whose ... [more]

Nat Commun Dec. 26, 2020; 12(1);1302 [Pubmed: 33637726]

Throughput

Low Throughput

Ontology Terms

growth abnormality (HP:0001507) [viability (PATO:0000169)]

Additional Notes

CRISPR GI screen
Cell Line: RPE-1
Experimental Setup: Timecourse
GIST: A-phenotypic negative genetic interaction
Library: Targeted CRISPR synthetic lethality library
Significance Threshold:FDR<0.1 and additional filtering of gene pairs with one gene with a large individual fitness defect

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
PARP1 RAD21	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	Go CD (2021)	High	16.56	BioGRID	2998654

Curated By

BioGRID

Interaction Summary

PARP1

Gene Ontology Biological Process

Gene Ontology Molecular Function

NAD+ ADP-ribosyltransferase activity [IDA, TAS]enzyme binding [IPI]identical protein binding [IPI]poly(A) RNA binding [IDA]protein N-terminus binding [IPI]protein binding [IPI]transcription factor binding [IPI]

Gene Ontology Cellular Component

RAD21