BAIT

TMEM185B

FAM11B
transmembrane protein 185B
GO Process (0)
GO Function (0)
GO Component (0)
Homo sapiens
PREY

TMEM185A

CXorf13, FAM11A, FRAXF, ee3
transmembrane protein 185A
GO Process (0)
GO Function (1)
GO Component (1)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

Combinatorial CRISPR screen identifies fitness effects of gene paralogues.

Thompson NA, Ranzani M, van der Weyden L, Iyer V, Offord V, Droop A, Behan F, Goncalves E, Speak A, Iorio F, Hewinson J, Harle V, Robertson H, Anderson E, Fu B, Yang F, Zagnoli-Vieira G, Chapman P, Del Castillo Velasco-Herrera M, Garnett MJ, Jackson SP, Adams DJ

Genetic redundancy has evolved as a way for human cells to survive the loss of genes that are single copy and essential in other organisms, but also allows tumours to survive despite having highly rearranged genomes. In this study we CRISPR screen 1191 gene pairs, including paralogues and known and predicted synthetic lethal interactions to identify 105 gene combinations whose ... [more]

Nat Commun Dec. 26, 2020; 12(1);1302 [Pubmed: 33637726]

Throughput

  • Low Throughput

Ontology Terms

  • growth abnormality (HP:0001507) [viability (PATO:0000169)]

Additional Notes

  • CRISPR GI screen
  • Cell Line: RPE-1
  • Experimental Setup: Timecourse
  • GIST: A-phenotypic negative genetic interaction
  • Library: Targeted CRISPR synthetic lethality library
  • Significance Threshold:FDR<0.1 and additional filtering of gene pairs with one gene with a large individual fitness defect

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
TMEM185A TMEM185B
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High1BioGRID
1189526
TMEM185A TMEM185B
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High1BioGRID
2221418
TMEM185A TMEM185B
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.9999BioGRID
3056465

Curated By

  • BioGRID