BAIT

NUP1

FG-nucleoporin NUP1, L000001288, YOR098C

FG-nucleoporin component of central core of the nuclear pore complex; contributes directly to nucleocytoplasmic transport and maintenance of thenuclear pore complex (NPC) permeability barrier; possible karyopherin release factor that accelerates release of karyopherin-cargo complexes after transport across NPC; both NUP1 and NUP60 are homologous to human NUP153

GO Process (5)

GO Function (2)

GO Component (4)

Gene Ontology Biological Process

NLS-bearing protein import into nucleus [IGI, IMP]

poly(A)+ mRNA export from nucleus [IMP]

protein import into nucleus [IMP]

ribosomal large subunit export from nucleus [IMP]

transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery [IMP]

Gene Ontology Molecular Function

nucleocytoplasmic transporter activity [IPI]
structural constituent of nuclear pore [IMP]

Gene Ontology Cellular Component

integral component of membrane [ISM]

nuclear pore [IDA]

nuclear pore central transport channel [IDA]

nuclear pore nuclear basket [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

SRP1

KAP60, SCM1, karyopherin alpha, L000002755, L000002780, YNL189W

Karyopherin alpha homolog; forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; involved in cotranslational protein degradation; binds ribosome-bound nascent polypeptides; Srp1p and Sts1p couple proteasomes to nascent polypeptides emerging from the ribosome for cotranslational degradation

GO Process (3)

GO Function (1)

GO Component (4)

Gene Ontology Biological Process

proteasome localization [IMP]

protein import into nucleus [IMP]

protein targeting to membrane [IMP]

Gene Ontology Molecular Function

protein transporter activity [IDA]

Gene Ontology Cellular Component

cytoplasm [IDA]

integral component of membrane [ISM]

nuclear exosome (RNase complex) [IDA]

nucleus [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Using affinity chromatography to investigate novel protein-protein interactions in an undergraduate cell and molecular biology lab course.

Belanger KD

Inquiry-driven lab exercises require students to think carefully about a question, carry out an investigation of that question, and critically analyze the results of their investigation. Here, we describe the implementation and assessment of an inquiry-based laboratory exercise in which students obtain and analyze novel data that contribute to our understanding of macromolecular trafficking between the nucleus and cytoplasm in ... [more]

CBE Life Sci Educ Sep. 03, 2009; 8(3);214-25 [Pubmed: 19723816]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
NUP1 SRP1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Alber F (2007)	Low	-	BioGRID	-
NUP1 SRP1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Allen NP (2001)	High	-	BioGRID	-
SRP1 NUP1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Gavin AC (2006)	High	-	BioGRID	-
NUP1 SRP1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Gavin AC (2006)	High	-	BioGRID	-
SRP1 NUP1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Ho Y (2002)	High	-	BioGRID	-
SRP1 NUP1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Krogan NJ (2006)	High	-	BioGRID	-
NUP1 SRP1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Lutzmann M (2005)	High	-	BioGRID	-
SRP1 NUP1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Michaelis AC (2023)	High	6	BioGRID	3598321
NUP1 SRP1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Belanger KD (1994)	Low	-	BioGRID	-
SRP1 NUP1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Belanger KD (1994)	Low	-	BioGRID	-
NUP1 SRP1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Meszaros N (2015)	Low	-	BioGRID	-
NUP1 SRP1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Booth JW (1999)	Low	-	BioGRID	-
NUP1 SRP1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Pyhtila B (2003)	Low	-	BioGRID	-
NUP1 SRP1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Solsbacher J (2000)	Low	-	BioGRID	-
NUP1 SRP1	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Belanger KD (1994)	Low	-	BioGRID	158401
NUP1 SRP1	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Harper NC (2008)	Low	-	BioGRID	264018

Curated By

BioGRID

Interaction Summary

NUP1

Gene Ontology Biological Process

Gene Ontology Molecular Function

nucleocytoplasmic transporter activity [IPI]structural constituent of nuclear pore [IMP]

Gene Ontology Cellular Component

SRP1

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein transporter activity [IDA]

Gene Ontology Cellular Component

Affinity Capture-Western

Publication

Using affinity chromatography to investigate novel protein-protein interactions in an undergraduate cell and molecular biology lab course.

Throughput

Related interactions

Curated By

nucleocytoplasmic transporter activity [IPI]
structural constituent of nuclear pore [IMP]