Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

Publication

Scalable multiplex co-fractionation/mass spectrometry platform for accelerated protein interactome discovery.

Havugimana PC, Goel RK, Phanse S, Youssef A, Padhorny D, Kotelnikov S, Kozakov D, Emili A

Co-fractionation/mass spectrometry (CF/MS) enables the mapping of endogenous macromolecular networks on a proteome scale, but current methods are experimentally laborious, resource intensive and afford lesser quantitative accuracy. Here, we present a technically efficient, cost-effective and reproducible multiplex CF/MS (mCF/MS) platform for measuring and comparing, simultaneously, multi-protein assemblies across different experimental samples at a rate that is up to an order ... [more]

Nat Commun Jul. 13, 2022; 13(1);4043 [Pubmed: 35831314]

Throughput

High Throughput

Additional Notes

High confidence interactions were identified as having an EPIC score >=0.625 in applicable cell lines (MCF7, MDA231 or MCF10A)
MCF10A cell line (score 0.717)
MCF7 cell line (score 0.694)
MDA231 cell line (score 0.713)

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
SNX2 VPS29	Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.	Havugimana PC (2012)	High	0.859	BioGRID	745324
VPS29 SNX2	Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.	Kristensen AR (2012)	High	-	BioGRID	925730
SNX2 VPS29	Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.	Wan C (2015)	High	0.3752	BioGRID	1274418

Curated By

BioGRID

Interaction Summary

SNX2

Gene Ontology Biological Process

Gene Ontology Molecular Function

epidermal growth factor receptor binding [IDA]insulin receptor binding [IDA]leptin receptor binding [IDA]phosphatidylinositol binding [IBA]protein binding [IPI]transferrin receptor binding [IDA]

Gene Ontology Cellular Component

VPS29

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]protein transporter activity [IBA]zinc ion binding [IBA]