BAIT
TOP2A
TOP2, TP2A
topoisomerase (DNA) II alpha 170kDa
GO Process (15)
GO Function (16)
GO Component (9)
Gene Ontology Biological Process
- ATP catabolic process [IDA]
- DNA ligation [IDA]
- DNA topological change [IDA]
- DNA unwinding involved in DNA replication [IBA]
- apoptotic chromosome condensation [IDA]
- cellular response to DNA damage stimulus [IDA]
- chromosome segregation [IMP]
- mitotic DNA integrity checkpoint [IBA]
- mitotic cell cycle [TAS]
- mitotic recombination [IBA]
- positive regulation of apoptotic process [IDA]
- positive regulation of single stranded viral RNA replication via double stranded DNA intermediate [IMP]
- positive regulation of viral genome replication [IMP]
- resolution of meiotic recombination intermediates [IBA]
- sister chromatid segregation [IBA]
Gene Ontology Molecular Function- DNA binding [IDA]
- DNA binding, bending [IDA]
- DNA topoisomerase type II (ATP-hydrolyzing) activity [IDA]
- DNA-dependent ATPase activity [IDA]
- chromatin binding [IDA]
- drug binding [IDA]
- enzyme binding [IPI]
- histone deacetylase binding [IPI]
- magnesium ion binding [IDA]
- poly(A) RNA binding [IDA]
- protein C-terminus binding [IPI]
- protein binding [IPI]
- protein heterodimerization activity [IPI]
- protein homodimerization activity [IPI]
- protein kinase C binding [IPI]
- ubiquitin binding [IMP]
- DNA binding [IDA]
- DNA binding, bending [IDA]
- DNA topoisomerase type II (ATP-hydrolyzing) activity [IDA]
- DNA-dependent ATPase activity [IDA]
- chromatin binding [IDA]
- drug binding [IDA]
- enzyme binding [IPI]
- histone deacetylase binding [IPI]
- magnesium ion binding [IDA]
- poly(A) RNA binding [IDA]
- protein C-terminus binding [IPI]
- protein binding [IPI]
- protein heterodimerization activity [IPI]
- protein homodimerization activity [IPI]
- protein kinase C binding [IPI]
- ubiquitin binding [IMP]
Gene Ontology Cellular Component
Homo sapiens
PREY
DYNC1H1
DHC1, DHC1a, DNCH1, DNCL, DNECL, DYHC, Dnchc1, HL-3, SMALED1, p22
dynein, cytoplasmic 1, heavy chain 1
GO Process (6)
GO Function (2)
GO Component (6)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Co-fractionation
Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.
Publication
Scalable multiplex co-fractionation/mass spectrometry platform for accelerated protein interactome discovery.
Co-fractionation/mass spectrometry (CF/MS) enables the mapping of endogenous macromolecular networks on a proteome scale, but current methods are experimentally laborious, resource intensive and afford lesser quantitative accuracy. Here, we present a technically efficient, cost-effective and reproducible multiplex CF/MS (mCF/MS) platform for measuring and comparing, simultaneously, multi-protein assemblies across different experimental samples at a rate that is up to an order ... [more]
Nat Commun Jul. 13, 2022; 13(1);4043 [Pubmed: 35831314]
Throughput
- High Throughput
Additional Notes
- High confidence interactions were identified as having an EPIC score >=0.625 in applicable cell lines (MCF7, MDA231 or MCF10A)
- MCF10A cell line (score 0.699)
- MCF7 cell line (score 0.705)
- MDA231 cell line (score 0.72)
Curated By
- BioGRID