CLB5
Gene Ontology Biological Process
- G1/S transition of mitotic cell cycle [IEP, IMP]
- G2/M transition of mitotic cell cycle [IEP, IMP]
- positive regulation of DNA replication [IMP]
- positive regulation of spindle pole body separation [IGI]
- premeiotic DNA replication [IGI, IMP]
- regulation of cyclin-dependent protein serine/threonine kinase activity [IDA]
- spindle assembly [IGI, IMP]
Gene Ontology Molecular Function
BUB3
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Synthetic Lethality
A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.
Publication
Specific genetic interactions between spindle assembly checkpoint proteins and B-Type cyclins in Saccharomyces cerevisiae.
The B-type cyclin Clb5 is involved primarily in control of DNA replication in Saccharomyces cerevisiae. We conducted a synthetic genetic array (SGA) analysis, testing for synthetic lethality between the clb5 deletion and a selected 87 deletions related to diverse aspects of cell cycle control based on GO annotations. Deletion of the spindle checkpoint genes BUB1 and BUB3 caused synthetic lethality ... [more]
Throughput
- Low Throughput
Ontology Terms
- phenotype: inviable (APO:0000112)
- phenotype: heat sensitivity (APO:0000147)
- phenotype: cytokinesis (APO:0000071)
- phenotype: spindle morphology (APO:0000213)
Additional Notes
- Low Throughput: The modified SGA analysis involved a clb5 deletion mutant as the query strain. The temperature-sensitive clb5-ts bub3 double mutant showed defects in spindle elongation and cytokinesis at 37 degrees Celsius.
- The results from the modified small-scale SGA analysis were confirmed by tetrad analysis.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| BUB3 CLB5 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.376 | BioGRID | 414280 | |
| CLB5 BUB3 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.4763 | BioGRID | 2196196 | |
| BUB3 CLB5 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.3773 | BioGRID | 2181885 | |
| BUB3 CLB5 | Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition. | High | - | BioGRID | 167545 | |
| BUB3 CLB5 | Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition. | Low | - | BioGRID | 3309658 |
Curated By
- BioGRID