BAIT

CLB4

B-type cyclin CLB4, L000000352, YLR210W
B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation; CLB4 has a paralog, CLB3, that arose from the whole genome duplication
Kinome Project (Sc)
Saccharomyces cerevisiae (S288c)
PREY

MAD2

spindle checkpoint protein MAD2, L000000975, YJL030W
Component of the spindle-assembly checkpoint complex; delays onset of anaphase in cells with defects in mitotic spindle assembly; forms a complex with Mad1p; regulates APC/C activity during prometaphase and metaphase of meiosis I; gene dosage imbalance between MAD1 and MAD2 leads to chromosome instability
GO Process (3)
GO Function (0)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

Specific genetic interactions between spindle assembly checkpoint proteins and B-Type cyclins in Saccharomyces cerevisiae.

Ikui AE, Cross FR

The B-type cyclin Clb5 is involved primarily in control of DNA replication in Saccharomyces cerevisiae. We conducted a synthetic genetic array (SGA) analysis, testing for synthetic lethality between the clb5 deletion and a selected 87 deletions related to diverse aspects of cell cycle control based on GO annotations. Deletion of the spindle checkpoint genes BUB1 and BUB3 caused synthetic lethality ... [more]

Genetics Sep. 01, 2009; 183(1);51-61 [Pubmed: 19581447]

Throughput

  • Low Throughput

Ontology Terms

  • spindle morphology (APO:0000213)
  • inviable (APO:0000112)

Additional Notes

  • The clb3 clb4 mad2 triple mutant has defects in spindle pole body separation and spindle assembly.
  • This is a clb3 clb4 mad2 triple mutant. MAD2 was found to be essential in the absence of CLB3 and CLB4.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
MAD2 CLB4
Phenotypic Enhancement
Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Low-BioGRID
3805283

Curated By

  • BioGRID