Gamma subunit of the translation initiation factor eIF2; involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met; mutations in human ortholog cause X-linked intellectual disability (XLID)

GO Process (3)

GO Function (5)

GO Component (4)

Gene Ontology Biological Process

formation of translation preinitiation complex [IDA]

positive regulation of translational fidelity [IMP]

translational initiation [IMP]

Gene Ontology Molecular Function

GTP binding [IMP]
protein binding [IPI]
tRNA binding [IMP]
translation initiation factor activity [IMP]
translation initiation factor binding [IDA]

Gene Ontology Cellular Component

eukaryotic 43S preinitiation complex [IDA]

eukaryotic 48S preinitiation complex [IDA]

eukaryotic translation initiation factor 2 complex [IMP]

multi-eIF complex [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Conserved sequences in the beta subunit of archaeal and eukaryal translation initiation factor 2 (eIF2), absent from eIF5, mediate interaction with eIF2gamma.

Thompson GM, Pacheco E, Melo EO, Castilho BA

The eukaryotic translation initiation factor 2 (eIF2) binds the methionyl-initiator tRNA in a GTP-dependent mode. This complex associates with the 40 S ribosomal particle, which then, with the aid of other factors, binds to the 5' end of the mRNA and migrates to the first AUG codon, where eIF5 promotes GTP hydrolysis, followed by the formation of the 80 S ... [more]

Biochem. J. May. 01, 2000; 347(0);703-9 [Pubmed: 10769173]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
GCD11 SUI3	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Gavin AC (2006)	High	-	BioGRID	-
GCD11 SUI3	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Gordiyenko Y (2014)	Low	-	BioGRID	-
GCD11 SUI3	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Krogan NJ (2004)	High	-	BioGRID	-
GCD11 SUI3	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Michaelis AC (2023)	High	2	BioGRID	3617712
GCD11 SUI3	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Alone PV (2008)	Low	-	BioGRID	-
SUI3 GCD11	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Dev K (2010)	Low	-	BioGRID	-
GCD11 SUI3	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Perzlmaier AF (2013)	Low	-	BioGRID	1174484
SUI3 GCD11	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Perzlmaier AF (2013)	Low	-	BioGRID	1174487
SUI3 GCD11	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Singh CR (2005)	Low	-	BioGRID	-
GCD11 SUI3	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Vanselow S (2022)	Low	-	BioGRID	-
SUI3 GCD11	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Vanselow S (2022)	Low	-	BioGRID	-
GCD11 SUI3	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Alone PV (2006)	Low	-	BioGRID	-
SUI3 GCD11	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Alone PV (2006)	Low	-	BioGRID	-
SUI3 GCD11	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Gordiyenko Y (2014)	Low	-	BioGRID	2862968
SUI3 GCD11	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Hashimoto NN (2002)	Low	-	BioGRID	-
GCD11 SUI3	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Hashimoto NN (2002)	Low	-	BioGRID	-
SUI3 GCD11	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Perzlmaier AF (2013)	Low	-	BioGRID	1174495
GCD11 SUI3	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Thompson GM (2000)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

SUI3

Gene Ontology Biological Process

Gene Ontology Molecular Function

mRNA binding [IDA]translation initiation factor activity [IMP]translation initiation factor binding [IDA, IPI]

Gene Ontology Cellular Component

GCD11

Gene Ontology Biological Process

Gene Ontology Molecular Function

GTP binding [IMP]protein binding [IPI]tRNA binding [IMP]translation initiation factor activity [IMP]translation initiation factor binding [IDA]

Gene Ontology Cellular Component

Affinity Capture-Western

Publication

Conserved sequences in the beta subunit of archaeal and eukaryal translation initiation factor 2 (eIF2), absent from eIF5, mediate interaction with eIF2gamma.

Throughput

Related interactions

Curated By

mRNA binding [IDA]
translation initiation factor activity [IMP]
translation initiation factor binding [IDA, IPI]

GTP binding [IMP]
protein binding [IPI]
tRNA binding [IMP]
translation initiation factor activity [IMP]
translation initiation factor binding [IDA]